SrasV1, Main, Exploration, bibRecord, 001C43

The Adjuvanticity of an O. volvulus-Derived rOv-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates

Identifieur interne : 001C43 ( Main/Exploration ); précédent : 001C42; suivant : 001C44

The Adjuvanticity of an O. volvulus-Derived rOv-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates

Auteurs : Jing Wang [États-Unis] ; Nancy Tricoche [États-Unis] ; Lanying Du [États-Unis] ; Meredith Hunter [États-Unis] ; Bin Zhan [États-Unis] ; Gaddam Goud [États-Unis] ; Elizabeth S. Didier [États-Unis] ; Jing Liu [États-Unis] ; Lu Lu [États-Unis] ; Preston A. Marx [États-Unis] ; Shibo Jiang [États-Unis] ; Sara Lustigman [États-Unis]

Source :

PLoS ONE [ 1932-6203 ] ; 2012.

RBID : PMC:3355165

Descripteurs français

KwdFr :
- Adjuvants immunologiques (pharmacologie), Animaux, Antigènes d'helminthe (immunologie), Cellules HEK293, Femelle, Humains, Hémagglutinines (immunologie), Onchocerca volvulus (immunologie), Onchocerca volvulus (métabolisme), Primates (immunologie), Production d'anticorps, Protéines d'helminthes (immunologie), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Vaccination (), Vaccins antigrippaux (immunologie), Virus du SRAS (immunologie).
MESH :
- immunologie : Antigènes d'helminthe, Hémagglutinines, Onchocerca volvulus, Primates, Protéines d'helminthes, Protéines recombinantes, Vaccins antigrippaux, Virus du SRAS.
- métabolisme : Onchocerca volvulus.
- pharmacologie : Adjuvants immunologiques.
- Animaux, Cellules HEK293, Femelle, Humains, Production d'anticorps, Souris, Souris de lignée BALB C, Vaccination.

English descriptors

KwdEn :
- Adjuvants, Immunologic (pharmacology), Animals, Antibody Formation, Antigens, Helminth (immunology), Female, HEK293 Cells, Helminth Proteins (immunology), Hemagglutinins (immunology), Humans, Influenza Vaccines (immunology), Mice, Mice, Inbred BALB C, Onchocerca volvulus (immunology), Onchocerca volvulus (metabolism), Primates (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Vaccination (methods).
MESH :
- chemical , immunology : Antigens, Helminth, Helminth Proteins, Hemagglutinins, Influenza Vaccines, Recombinant Proteins.
- chemical , pharmacology : Adjuvants, Immunologic.
- immunology : Onchocerca volvulus, Primates, SARS Virus.
- metabolism : Onchocerca volvulus.
- methods : Vaccination.
- Animals, Antibody Formation, Female, HEK293 Cells, Humans, Mice, Mice, Inbred BALB C.

Abstract

Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the Onchocerca volvulus recombinant protein rOv-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6. However, because it is a protein, the rOv-ASP-1 adjuvant may also induce anti-self-antibodies. Therefore, it was important to verify that the host responses to self will not affect the adjuvanticity of rOv-ASP-1 when it is used in subsequent vaccinations with the same or different vaccine antigens. In this study, we have established rOv-ASP-1's adjuvanticity in mice during the course of two sequential vaccinations using two vaccine model systems: the receptor-binding domain (RBD) of SARS-CoV spike protein and a commercial influenza virus hemagglutinin (HA) vaccine comprised of three virus strains. Moreover, the adjuvanticity of rOv-ASP-1 was retained with an efficacy similar to that obtained when it was used for a first vaccination, even though a high level of anti-rOv-ASP-1 antibodies was present in the sera of mice before the administration of the second vaccine. To further demonstrate its utility as an adjuvant for human use, we also immunized non-human primates (NHPs) with RBD plus rOv-ASP-1 and showed that rOv-ASP-1 could induce high titres of functional and protective anti-RBD antibody responses in NHPs. Notably, the rOv-ASP-1 adjuvant did not induce high titer antibodies against self in NHPs. Thus, the present study provided a sound scientific foundation for future strategies in the development of this novel protein adjuvant.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3355165

DOI: 10.1371/journal.pone.0037019
PubMed: 22615877
PubMed Central: 3355165

Affiliations:

Le document en format XML

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-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates</title>
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-Derived r<italic>Ov</italic>
-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates</title>
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</nlm:aff>
<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Goud, Gaddam" sort="Goud, Gaddam" uniqKey="Goud G" first="Gaddam" last="Goud">Gaddam Goud</name>
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</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, Immunology and Tropical Medicine, George Washington University and the Sabin Vaccine Institute, Washington, D.C.</wicri:regionArea>
<wicri:noRegion>D.C.</wicri:noRegion>
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<author><name sortKey="Didier, Elizabeth S" sort="Didier, Elizabeth S" uniqKey="Didier E" first="Elizabeth S." last="Didier">Elizabeth S. Didier</name>
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<author><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name>
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<author><name sortKey="Lu, Lu" sort="Lu, Lu" uniqKey="Lu L" first="Lu" last="Lu">Lu Lu</name>
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</nlm:aff>
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<placeName><region type="state">État de New York</region>
</placeName>
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<author><name sortKey="Marx, Preston A" sort="Marx, Preston A" uniqKey="Marx P" first="Preston A." last="Marx">Preston A. Marx</name>
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<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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</nlm:aff>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (pharmacology)</term>
<term>Animals</term>
<term>Antibody Formation</term>
<term>Antigens, Helminth (immunology)</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>Helminth Proteins (immunology)</term>
<term>Hemagglutinins (immunology)</term>
<term>Humans</term>
<term>Influenza Vaccines (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Onchocerca volvulus (immunology)</term>
<term>Onchocerca volvulus (metabolism)</term>
<term>Primates (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Vaccination (methods)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (pharmacologie)</term>
<term>Animaux</term>
<term>Antigènes d'helminthe (immunologie)</term>
<term>Cellules HEK293</term>
<term>Femelle</term>
<term>Humains</term>
<term>Hémagglutinines (immunologie)</term>
<term>Onchocerca volvulus (immunologie)</term>
<term>Onchocerca volvulus (métabolisme)</term>
<term>Primates (immunologie)</term>
<term>Production d'anticorps</term>
<term>Protéines d'helminthes (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Vaccination ()</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term>
<term>Helminth Proteins</term>
<term>Hemagglutinins</term>
<term>Influenza Vaccines</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term>
<term>Hémagglutinines</term>
<term>Onchocerca volvulus</term>
<term>Primates</term>
<term>Protéines d'helminthes</term>
<term>Protéines recombinantes</term>
<term>Vaccins antigrippaux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Onchocerca volvulus</term>
<term>Primates</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Onchocerca volvulus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Onchocerca volvulus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adjuvants immunologiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Antibody Formation</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules HEK293</term>
<term>Femelle</term>
<term>Humains</term>
<term>Production d'anticorps</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Vaccination</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en"><p>Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the <italic>Onchocerca volvulus</italic>
 recombinant protein r<italic>Ov</italic>
-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6. However, because it is a protein, the r<italic>Ov</italic>
-ASP-1 adjuvant may also induce anti-self-antibodies. Therefore, it was important to verify that the host responses to self will not affect the adjuvanticity of r<italic>Ov</italic>
-ASP-1 when it is used in subsequent vaccinations with the same or different vaccine antigens. In this study, we have established r<italic>Ov</italic>
-ASP-1's adjuvanticity in mice during the course of two sequential vaccinations using two vaccine model systems: the receptor-binding domain (RBD) of SARS-CoV spike protein and a commercial influenza virus hemagglutinin (HA) vaccine comprised of three virus strains. Moreover, the adjuvanticity of r<italic>Ov</italic>
-ASP-1 was retained with an efficacy similar to that obtained when it was used for a first vaccination, even though a high level of anti-r<italic>Ov</italic>
-ASP-1 antibodies was present in the sera of mice before the administration of the second vaccine. To further demonstrate its utility as an adjuvant for human use, we also immunized non-human primates (NHPs) with RBD plus r<italic>Ov</italic>
-ASP-1 and showed that r<italic>Ov</italic>
-ASP-1 could induce high titres of functional and protective anti-RBD antibody responses in NHPs. Notably, the r<italic>Ov</italic>
-ASP-1 adjuvant did not induce high titer antibodies against self in NHPs. Thus, the present study provided a sound scientific foundation for future strategies in the development of this novel protein adjuvant.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mbow, Ml" uniqKey="Mbow M">ML Mbow</name>
</author>
<author><name sortKey="De Gregorio, E" uniqKey="De Gregorio E">E De Gregorio</name>
</author>
<author><name sortKey="Ulmer, Jb" uniqKey="Ulmer J">JB Ulmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Flach, Tl" uniqKey="Flach T">TL Flach</name>
</author>
<author><name sortKey="Ng, G" uniqKey="Ng G">G Ng</name>
</author>
<author><name sortKey="Hari, A" uniqKey="Hari A">A Hari</name>
</author>
<author><name sortKey="Desrosiers, Md" uniqKey="Desrosiers M">MD Desrosiers</name>
</author>
<author><name sortKey="Zhang, P" uniqKey="Zhang P">P Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pulendran, B" uniqKey="Pulendran B">B Pulendran</name>
</author>
<author><name sortKey="Ahmed, R" uniqKey="Ahmed R">R Ahmed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Coffman, Rl" uniqKey="Coffman R">RL Coffman</name>
</author>
<author><name sortKey="Sher, A" uniqKey="Sher A">A Sher</name>
</author>
<author><name sortKey="Seder, Ra" uniqKey="Seder R">RA Seder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kenney, Rt" uniqKey="Kenney R">RT Kenney</name>
</author>
<author><name sortKey="Cross, As" uniqKey="Cross A">AS Cross</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singh, M" uniqKey="Singh M">M Singh</name>
</author>
<author><name sortKey="O Hagan, Dt" uniqKey="O Hagan D">DT O'Hagan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hauguel, Tm" uniqKey="Hauguel T">TM Hauguel</name>
</author>
<author><name sortKey="Hackett, Cj" uniqKey="Hackett C">CJ Hackett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Azizi, A" uniqKey="Azizi A">A Azizi</name>
</author>
<author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name>
</author>
<author><name sortKey="Diaz Mitoma, F" uniqKey="Diaz Mitoma F">F Diaz-Mitoma</name>
</author>
<author><name sortKey="Mestecky, J" uniqKey="Mestecky J">J Mestecky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Allen, Je" uniqKey="Allen J">JE Allen</name>
</author>
<author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
<author><name sortKey="Yazdanbakhsh, M" uniqKey="Yazdanbakhsh M">M Yazdanbakhsh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jarnicki, Ag" uniqKey="Jarnicki A">AG Jarnicki</name>
</author>
<author><name sortKey="Fallon, Pg" uniqKey="Fallon P">PG Fallon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kane, Cm" uniqKey="Kane C">CM Kane</name>
</author>
<author><name sortKey="Cervi, L" uniqKey="Cervi L">L Cervi</name>
</author>
<author><name sortKey="Sun, J" uniqKey="Sun J">J Sun</name>
</author>
<author><name sortKey="Mckee, As" uniqKey="Mckee A">AS McKee</name>
</author>
<author><name sortKey="Masek, Ks" uniqKey="Masek K">KS Masek</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yarovinsky, F" uniqKey="Yarovinsky F">F Yarovinsky</name>
</author>
<author><name sortKey="Zhang, D" uniqKey="Zhang D">D Zhang</name>
</author>
<author><name sortKey="Andersen, Jf" uniqKey="Andersen J">JF Andersen</name>
</author>
<author><name sortKey="Bannenberg, Gl" uniqKey="Bannenberg G">GL Bannenberg</name>
</author>
<author><name sortKey="Serhan, Cn" uniqKey="Serhan C">CN Serhan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Macdonald, Aj" uniqKey="Macdonald A">AJ MacDonald</name>
</author>
<author><name sortKey="Cao, L" uniqKey="Cao L">L Cao</name>
</author>
<author><name sortKey="He, Y" uniqKey="He Y">Y He</name>
</author>
<author><name sortKey="Zhao, Q" uniqKey="Zhao Q">Q Zhao</name>
</author>
<author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
<author><name sortKey="Gomez Escobar, N" uniqKey="Gomez Escobar N">N Gomez-Escobar</name>
</author>
<author><name sortKey="Gregory, Wf" uniqKey="Gregory W">WF Gregory</name>
</author>
<author><name sortKey="Murray, J" uniqKey="Murray J">J Murray</name>
</author>
<author><name sortKey="Zang, X" uniqKey="Zang X">X Zang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
<author><name sortKey="Balic, A" uniqKey="Balic A">A Balic</name>
</author>
<author><name sortKey="Gomez Escobar, N" uniqKey="Gomez Escobar N">N Gomez-Escobar</name>
</author>
<author><name sortKey="Nair, M" uniqKey="Nair M">M Nair</name>
</author>
<author><name sortKey="Taylor, Md" uniqKey="Taylor M">MD Taylor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcinnes, Ib" uniqKey="Mcinnes I">IB McInnes</name>
</author>
<author><name sortKey="Leung, Bp" uniqKey="Leung B">BP Leung</name>
</author>
<author><name sortKey="Harnett, M" uniqKey="Harnett M">M Harnett</name>
</author>
<author><name sortKey="Gracie, Ja" uniqKey="Gracie J">JA Gracie</name>
</author>
<author><name sortKey="Liew, Fy" uniqKey="Liew F">FY Liew</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Harnett, W" uniqKey="Harnett W">W Harnett</name>
</author>
<author><name sortKey="Mcinnes, Ib" uniqKey="Mcinnes I">IB McInnes</name>
</author>
<author><name sortKey="Harnett, Mm" uniqKey="Harnett M">MM Harnett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Paterson, Jc" uniqKey="Paterson J">JC Paterson</name>
</author>
<author><name sortKey="Garside, P" uniqKey="Garside P">P Garside</name>
</author>
<author><name sortKey="Kennedy, Mw" uniqKey="Kennedy M">MW Kennedy</name>
</author>
<author><name sortKey="Lawrence, Ce" uniqKey="Lawrence C">CE Lawrence</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dissanayake, S" uniqKey="Dissanayake S">S Dissanayake</name>
</author>
<author><name sortKey="Amith, Rs" uniqKey="Amith R">RS Amith</name>
</author>
<author><name sortKey="Shahin, A" uniqKey="Shahin A">A Shahin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der Kleij, D" uniqKey="Van Der Kleij D">D van der Kleij</name>
</author>
<author><name sortKey="Latz, E" uniqKey="Latz E">E Latz</name>
</author>
<author><name sortKey="Brouwers, Jf" uniqKey="Brouwers J">JF Brouwers</name>
</author>
<author><name sortKey="Kruize, Yc" uniqKey="Kruize Y">YC Kruize</name>
</author>
<author><name sortKey="Schmitz, M" uniqKey="Schmitz M">M Schmitz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holland, Mj" uniqKey="Holland M">MJ Holland</name>
</author>
<author><name sortKey="Harcus, Ym" uniqKey="Harcus Y">YM Harcus</name>
</author>
<author><name sortKey="Riches, Pl" uniqKey="Riches P">PL Riches</name>
</author>
<author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Balic, A" uniqKey="Balic A">A Balic</name>
</author>
<author><name sortKey="Harcus, Y" uniqKey="Harcus Y">Y Harcus</name>
</author>
<author><name sortKey="Holland, Mj" uniqKey="Holland M">MJ Holland</name>
</author>
<author><name sortKey="Maizels, Rm" uniqKey="Maizels R">RM Maizels</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Okano, M" uniqKey="Okano M">M Okano</name>
</author>
<author><name sortKey="Satoskar, Ar" uniqKey="Satoskar A">AR Satoskar</name>
</author>
<author><name sortKey="Nishizaki, K" uniqKey="Nishizaki K">K Nishizaki</name>
</author>
<author><name sortKey="Harn Da" uniqKey="Harn Da">Harn DA</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thomas, Pg" uniqKey="Thomas P">PG Thomas</name>
</author>
<author><name sortKey="Carter, Mr" uniqKey="Carter M">MR Carter</name>
</author>
<author><name sortKey="Atochina, O" uniqKey="Atochina O">O Atochina</name>
</author>
<author><name sortKey="Da Dara, Aa" uniqKey="Da Dara A">AA Da'Dara</name>
</author>
<author><name sortKey="Piskorska, D" uniqKey="Piskorska D">D Piskorska</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Segura Velazquez, R" uniqKey="Segura Velazquez R">R Segura-Velazquez</name>
</author>
<author><name sortKey="Torres Perez, A" uniqKey="Torres Perez A">A Torres-Perez</name>
</author>
<author><name sortKey="Rosas, G" uniqKey="Rosas G">G Rosas</name>
</author>
<author><name sortKey="Toledo, A" uniqKey="Toledo A">A Toledo</name>
</author>
<author><name sortKey="Restelli, M" uniqKey="Restelli M">M Restelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, Y" uniqKey="He Y">Y He</name>
</author>
<author><name sortKey="Barker, Sj" uniqKey="Barker S">SJ Barker</name>
</author>
<author><name sortKey="Macdonald, Aj" uniqKey="Macdonald A">AJ MacDonald</name>
</author>
<author><name sortKey="Yu, Y" uniqKey="Yu Y">Y Yu</name>
</author>
<author><name sortKey="Cao, L" uniqKey="Cao L">L Cao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name>
</author>
<author><name sortKey="Du, L" uniqKey="Du L">L Du</name>
</author>
<author><name sortKey="Xiao, W" uniqKey="Xiao W">W Xiao</name>
</author>
<author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name>
</author>
<author><name sortKey="Lin, Y" uniqKey="Lin Y">Y Lin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xiao, W" uniqKey="Xiao W">W Xiao</name>
</author>
<author><name sortKey="Du, L" uniqKey="Du L">L Du</name>
</author>
<author><name sortKey="Liang, C" uniqKey="Liang C">C Liang</name>
</author>
<author><name sortKey="Guan, J" uniqKey="Guan J">J Guan</name>
</author>
<author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Macdonald, Aj" uniqKey="Macdonald A">AJ MacDonald</name>
</author>
<author><name sortKey="Libri, Na" uniqKey="Libri N">NA Libri</name>
</author>
<author><name sortKey="Lustigman, S" uniqKey="Lustigman S">S Lustigman</name>
</author>
<author><name sortKey="Barker, Sj" uniqKey="Barker S">SJ Barker</name>
</author>
<author><name sortKey="Whelan, Ma" uniqKey="Whelan M">MA Whelan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name>
</author>
<author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name>
</author>
<author><name sortKey="Lin, Y" uniqKey="Lin Y">Y Lin</name>
</author>
<author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name>
</author>
<author><name sortKey="He, Y" uniqKey="He Y">Y He</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name>
</author>
<author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name>
</author>
<author><name sortKey="Lin, Y" uniqKey="Lin Y">Y Lin</name>
</author>
<author><name sortKey="Sui, H" uniqKey="Sui H">H Sui</name>
</author>
<author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Teleshova, N" uniqKey="Teleshova N">N Teleshova</name>
</author>
<author><name sortKey="Kenney, J" uniqKey="Kenney J">J Kenney</name>
</author>
<author><name sortKey="Van Nest, G" uniqKey="Van Nest G">G Van Nest</name>
</author>
<author><name sortKey="Marshall, J" uniqKey="Marshall J">J Marshall</name>
</author>
<author><name sortKey="Lifson, Jd" uniqKey="Lifson J">JD Lifson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Teleshova, N" uniqKey="Teleshova N">N Teleshova</name>
</author>
<author><name sortKey="Kenney, J" uniqKey="Kenney J">J Kenney</name>
</author>
<author><name sortKey="Williams, V" uniqKey="Williams V">V Williams</name>
</author>
<author><name sortKey="Van Nest, G" uniqKey="Van Nest G">G Van Nest</name>
</author>
<author><name sortKey="Marshall, J" uniqKey="Marshall J">J Marshall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delavallee, L" uniqKey="Delavallee L">L Delavallee</name>
</author>
<author><name sortKey="Duvallet, E" uniqKey="Duvallet E">E Duvallet</name>
</author>
<author><name sortKey="Semerano, L" uniqKey="Semerano L">L Semerano</name>
</author>
<author><name sortKey="Assier, E" uniqKey="Assier E">E Assier</name>
</author>
<author><name sortKey="Boissier, Mc" uniqKey="Boissier M">MC Boissier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Myc, La" uniqKey="Myc L">LA Myc</name>
</author>
<author><name sortKey="Gamian, A" uniqKey="Gamian A">A Gamian</name>
</author>
<author><name sortKey="Myc, A" uniqKey="Myc A">A Myc</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schijns, Ve" uniqKey="Schijns V">VE Schijns</name>
</author>
<author><name sortKey="Lavelle, Ec" uniqKey="Lavelle E">EC Lavelle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Palucka, K" uniqKey="Palucka K">K Palucka</name>
</author>
<author><name sortKey="Ueno, H" uniqKey="Ueno H">H Ueno</name>
</author>
<author><name sortKey="Banchereau, J" uniqKey="Banchereau J">J Banchereau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, Jc" uniqKey="Cox J">JC Cox</name>
</author>
<author><name sortKey="Coulter, Ar" uniqKey="Coulter A">AR Coulter</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benko, S" uniqKey="Benko S">S Benko</name>
</author>
<author><name sortKey="Magyarics, Z" uniqKey="Magyarics Z">Z Magyarics</name>
</author>
<author><name sortKey="Szabo, A" uniqKey="Szabo A">A Szabo</name>
</author>
<author><name sortKey="Rajnavolgyi, E" uniqKey="Rajnavolgyi E">E Rajnavolgyi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vasilakos, Jp" uniqKey="Vasilakos J">JP Vasilakos</name>
</author>
<author><name sortKey="Smith, Rm" uniqKey="Smith R">RM Smith</name>
</author>
<author><name sortKey="Gibson, Sj" uniqKey="Gibson S">SJ Gibson</name>
</author>
<author><name sortKey="Lindh, Jm" uniqKey="Lindh J">JM Lindh</name>
</author>
<author><name sortKey="Pederson, Lk" uniqKey="Pederson L">LK Pederson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pashine, A" uniqKey="Pashine A">A Pashine</name>
</author>
<author><name sortKey="Valiante, Nm" uniqKey="Valiante N">NM Valiante</name>
</author>
<author><name sortKey="Ulmer, Jb" uniqKey="Ulmer J">JB Ulmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huleatt, Jw" uniqKey="Huleatt J">JW Huleatt</name>
</author>
<author><name sortKey="Jacobs, Ar" uniqKey="Jacobs A">AR Jacobs</name>
</author>
<author><name sortKey="Tang, J" uniqKey="Tang J">J Tang</name>
</author>
<author><name sortKey="Desai, P" uniqKey="Desai P">P Desai</name>
</author>
<author><name sortKey="Kopp, Eb" uniqKey="Kopp E">EB Kopp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcdonald, Wf" uniqKey="Mcdonald W">WF McDonald</name>
</author>
<author><name sortKey="Huleatt, Jw" uniqKey="Huleatt J">JW Huleatt</name>
</author>
<author><name sortKey="Foellmer, Hg" uniqKey="Foellmer H">HG Foellmer</name>
</author>
<author><name sortKey="Hewitt, D" uniqKey="Hewitt D">D Hewitt</name>
</author>
<author><name sortKey="Tang, J" uniqKey="Tang J">J Tang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kwissa, M" uniqKey="Kwissa M">M Kwissa</name>
</author>
<author><name sortKey="Kasturi, Sp" uniqKey="Kasturi S">SP Kasturi</name>
</author>
<author><name sortKey="Pulendran, B" uniqKey="Pulendran B">B Pulendran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marciani, Dj" uniqKey="Marciani D">DJ Marciani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lindblad, Eb" uniqKey="Lindblad E">EB Lindblad</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marchand, M" uniqKey="Marchand M">M Marchand</name>
</author>
<author><name sortKey="Punt, Cj" uniqKey="Punt C">CJ Punt</name>
</author>
<author><name sortKey="Aamdal, S" uniqKey="Aamdal S">S Aamdal</name>
</author>
<author><name sortKey="Escudier, B" uniqKey="Escudier B">B Escudier</name>
</author>
<author><name sortKey="Kruit, Wh" uniqKey="Kruit W">WH Kruit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huleatt, Jw" uniqKey="Huleatt J">JW Huleatt</name>
</author>
<author><name sortKey="Nakaar, V" uniqKey="Nakaar V">V Nakaar</name>
</author>
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<author><name sortKey="He, Y" uniqKey="He Y">Y He</name>
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<author><name sortKey="Zhan, B" uniqKey="Zhan B">B Zhan</name>
</author>
<author><name sortKey="Mendez, S" uniqKey="Mendez S">S Mendez</name>
</author>
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</author>
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</author>
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</author>
<author><name sortKey="Chan, Cc" uniqKey="Chan C">CC Chan</name>
</author>
<author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name>
</author>
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</author>
</analytic>
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</author>
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</author>
<author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name>
</author>
<author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name>
</author>
<author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name>
</author>
<author><name sortKey="Chan, Cc" uniqKey="Chan C">CC Chan</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L Li</name>
</author>
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</author>
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<affiliations><list><country><li>États-Unis</li>
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<region><li>Louisiane</li>
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<name sortKey="Didier, Elizabeth S" sort="Didier, Elizabeth S" uniqKey="Didier E" first="Elizabeth S." last="Didier">Elizabeth S. Didier</name>
<name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name>
<name sortKey="Goud, Gaddam" sort="Goud, Gaddam" uniqKey="Goud G" first="Gaddam" last="Goud">Gaddam Goud</name>
<name sortKey="Hunter, Meredith" sort="Hunter, Meredith" uniqKey="Hunter M" first="Meredith" last="Hunter">Meredith Hunter</name>
<name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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<name sortKey="Lu, Lu" sort="Lu, Lu" uniqKey="Lu L" first="Lu" last="Lu">Lu Lu</name>
<name sortKey="Lustigman, Sara" sort="Lustigman, Sara" uniqKey="Lustigman S" first="Sara" last="Lustigman">Sara Lustigman</name>
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<name sortKey="Zhan, Bin" sort="Zhan, Bin" uniqKey="Zhan B" first="Bin" last="Zhan">Bin Zhan</name>
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The Adjuvanticity of an O. volvulus-Derived rOv-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates

The Adjuvanticity of an O. volvulus-Derived rOv-ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates

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